Modern approaches to cryopreservation of mesenchymal stem cells

Authors

DOI:

https://doi.org/10.25305/unj.96083

Keywords:

mesenchymal stem cells, multipotent cells, cryopreservation, cryoprotectants, dimethyl sulfoxide

Abstract

The problem of treating inflammatory and degenerative lesions of the central nervous system remains relevant. In recent years, advances in the treatment of diseases are associated with the development of cellular technology. The umbilical cord stem cells exhibit the most promise for use in clinical practice. Its recovery does not raise any moral and ethical conflicts, and the culturing process is quite simple. The prospect of clinical application of human umbilical cord mesenchymal stem cells raises a question of choosing the optimal method for long-term storage (cryopreservation) considering freezing mode, type of cryoprotectants and storage time. This review focuses on the analysis of recent papers about cryopreservation of mesenchymal stem cells, including umbilical cord stem cells.

Author Biographies

Olga O. Maslova, Institute of Genetic and Regenerative Medicine of NAMS of Ukraine

Department of Gene Technology

Olena G. Deriabina, Institute of Genetic and Regenerative Medicine of NAMS of Ukraine

Department of Gene Technology

Leonid D. Pichkur, Romodanov Neurosurgery Institute

Restorative Neurosurgery Department

Svetlana A. Verbovska, Romodanov Neurosurgery Institute

Restorative Neurosurgery Department

Samuel T. Akinola, Romodanov Neurosurgery Institute

Restorative Neurosurgery Department

References

1. Tsymbaluk VI. [Neurotransplantation as a model for the future of medicine: ethical issues]. Mystetstvo likuvannya. 2004;(1):6-11. Ukrainian.

2. Fagioli F, Ferrero I. Mesenchymal stem cell manufacturing for clinical use. In: Taner Demirer, ed. Progress in Stem Cell Transplantation. 2015. [CrossRef]

3. Vangsness CT, Sternberg H, Harris L. Umbilical cord tissue offers the greatest number of harvestable mesenchymal stem cells for research and clinical application: а literature review of different harvest sites. Arthroscopy: J Arthroscop Relat Surg. 2015;31(9):1836-43. [CrossRef] [PubMed]

4. Maslova O, Novak M, Kruzliak P. Umbilical cord tissue-derived cells as therapeutic agents. Stem Cells International. 2015;2015:1-10. [CrossRef] [PubMed]

5. Balci D, Can A. The assessment of cryopreservation conditions for human umbilical cord stroma-derived mesenchymal stem cells towards a potential use for stem cell banking. Curr Stem Cell Res Ther. 2013;8(1):60-72. [CrossRef] [PubMed]

6. Asghar W, ElAssal R, Shafiee H, Anchan RM, Demirci U. Preserving human cells for regenerative, reproductive, and transfusion medicine. Biotechnol J. 2014;9(7):895-903. [CrossRef] [PubMed]

7. Mantri S, Kanungo S, Mohapatra PC. Cryoprotective effect of disaccharides on cord blood stem cells with minimal use of DMSO. Indian J Hematol Blood Transfus. 2015;31(2):206-12. [CrossRef] [PubMed]

8. Best BP. Cryoprotectant toxicity: facts, issues, and questions. Rejuvenation Res. 2015;18(5):422-36. [CrossRef] [PubMed]

9. Yong KW, WanSafwani WK, Xu F, WanAbas WA, Choi JR, Pingguan-Murphy B. Cryopreservation of human mesenchymal stem cells for clinical applications: current methods and challenges. Biopreservation and biobanking. 2015;13(4):231-9. [CrossRef] [PubMed]

10. Almansoori KA, Prasad V, Forbes JF, Law GK, McGann LE, Elliott JA, Jomha NM. Cryoprotective agent toxicity interactions in human articular chondrocytes. Cryobiology. 2012;64(3):185-91. [CrossRef] [PubMed]

11. Schnitzler AC, Verma A, Kehoe DE, Jing D, Murrell JR, Der KA, Aysola M, Rapiejko PJ, Punreddy S, Rook MS. Bioprocessing of human mesenchymal stem/stromal cells for therapeutic use: current technologies and challenges. Biochemical Engineering Journal. 2016;108:3-13. [CrossRef]

12. Chatzistamatiou TK, Papassavas AC, Michalopoulos E, Gamaloutsos C, Mallis P, Gontika I, Panagouli E, Koussoulakos SL, Stavropoulos-Giokas C. Optimizing isolation culture and freezing methods to preserve Wharton’s jelly’s mesenchymal stem cell (MSC) properties: an MSC banking protocol validation for the Hellenic Cord Blood Bank. Transfusion. 2014;54(12):3108-20. [CrossRef] [PubMed]

13. Davies OG, Smith AJ, Cooper PR, Shelton RM, Scheven BA. The effects of cryopreservation on cells isolated from adipose, bone marrow and dental pulp tissues. Cryobiology. 2014;69(2):342-7. [CrossRef] [PubMed]

14. Naaldijk Y, Fedorova V, Stolzing A. Cryopreservation of human umbilical cord-derived mesenchymal stem cells in complex sugar based cryoprotective solutions. Journal of Biotechnology Letters. 2013;4(2):95-9.

15. Buchanan SS, Gross SA, Acker JP, Toner M, Carpenter JF, Pyatt DW. Cryopreservation of stem cells using trehalose: evaluation of the method using a human hematopoietic cell line. Stem cells and development. 2004;13(3):295-305. [CrossRef] [PubMed]

16. Dovgan B, Dermol J, Barlič A, Kneћević M, Miklavčič D. Cryopreservation of Human Umbilical Stem Cells in Combination with Trehalose and Reversible Electroporation. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies 2016 (pp.307-310). Singapore: Springer. [CrossRef]

17. Hennes A, Gucciardo L, Zia S, Lesage F, Lefиvre N, Lewi L, Vorsselmans A, Cos T, Lories R, Deprest J, Toelen J. Safe and effective cryopreservation methods for long-term storage of human-amniotic-fluid-derived stem cells. Prenatal diagnosis. 2015;35(5):456-62. [CrossRef] [PubMed]

18. Al-Saqi SH, Saliem M, Quezada HC, Ekblad A, Jonasson AF, Hovatta O, Gцtherstrцm C. Defined serum- and xeno-free cryopreservation of mesenchymal stem cells. Cell and tissue banking. 2015;16(2):181-93. [CrossRef] [PubMed]

19. Kim S-M, Yun C-K, Park J-H, Hwang JW, Kim Z-H, Choi Y-S. Efficient cryopreservation of human mesenchymal stem cells using silkworm hemolymph-derived proteins. Journal of Tissue Engineering and Regenerative Medicine. 2016. [CrossRef]

20. Thirumala S, Fearnot E, Goebel WS, Woods EJ. A cryopreservation system for direct clinical use of MSC. Cytotherapy. 2013;15(4):46-7. [CrossRef]

21. Thirumala S, Goebel WS, Woods EJ. Manufacturing and banking of mesenchymal stem cells. Expert opinion on biological therapy. 2013;13(5):673-91. [CrossRef]

22. Fazzina R, Mariotti A, Procoli A, Fioravanti D, Iudicone P, Scambia G, Pierelli L, Bonanno G. A new standardized clinical grade protocol for banking human umbilical cord tissue cells. Transfusion. 2015;55(12):2864-73. [CrossRef] [PubMed]

23. Di Giuseppe F1, Pierdomenico L, Eleuterio E, Sulpizio M, Lanuti P, Riviello A, Bologna G, Gesi M, Di Ilio C, Miscia S, Marchisio M, Angelucci S. Cryopreservation effects on Wharton’s jelly stem cells proteome. Stem Cell Rev. 2014;10(3):429-46. [CrossRef] [PubMed]

24. Van Campenhout A, Swinnen L, Klykens J, Devos T, Verhoef G. Post-cryopreservation viability of mesenchymal stem cells. Cytotherapy. 2014;16(4):S83. [CrossRef]

25. Hoogduijn MJ, deWitte SF, Luk F, vanden Hout-van Vroonhoven MC, Ignatowicz L, Catar R, Strini T, Korevaar SS, van IJcken WF, Betjes MG, Franquesa M. Effects of freeze-thawing and intravenous infusion on mesenchymal stromal cell gene expression. Stem Cells Dev. 2016;25(8):586-97. [CrossRef] [PubMed]

26. Pollock K, Sumstad D, Kadidlo D, McKenna DH, Hubel A. Clinical mesenchymal stromal cell products undergo functional changes in response to freezing. Cytotherapy. 2015;17(1):38-45. [CrossRef] [PubMed]

27. Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, Sukhikh GT. Isolation of multipotent mesenchymal stroma cells from cryopreserved human umbilical cord tissue. Bull Exp Biol Med. 2016;160(4):530-4. [CrossRef] [PubMed]

28. Shimazu T, Mori Y, Takahashi A, Tsunoda H, Tojo A, Nagamura-Inoue T. Serum-and xeno-free cryopreservation of human umbilical cord tissue as mesenchymal stromal cell source. Cytotherapy. 2015;17(5):593-600. [CrossRef] [PubMed]

29. Singh P, Rose MK, Yadav PS, Sharma R, Singh J. Effects of cryoprotectants on freezing, culture behaviour of buffalo umbilical cord matrix cells. Journal of Cell and Tissue Research. 2013;13(2):3625-30.

30. Wong CY, Ooi GC, Vivian Kong WP, Chai MF, Thiagarajah K, Then KY, Cheong S. Mesenchymal stem cells derived from human umbilical cord and adipose tissue retain their properties after 24 months of cryopreservation. Cytotherapy. 2015;17(6):37–8. [CrossRef]

Downloads

Published

2017-03-17

How to Cite

Maslova, O. O., Deriabina, O. G., Pichkur, L. D., Verbovska, S. A., & Akinola, S. T. (2017). Modern approaches to cryopreservation of mesenchymal stem cells. Ukrainian Neurosurgical Journal, (1), 5–10. https://doi.org/10.25305/unj.96083

Issue

No. 1 (2017)

Section

Review articles

License

Copyright (c) 2017 Olga O. Maslova, Olena G. Deriabina, Leonid D. Pichkur, Svetlana A. Verbovska, Samuel T. Akinola

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Ukrainian Neurosurgical Journal abides by the  CREATIVE COMMONS copyright rights and permissions for open access journals.

Authors, who are published in this Journal, agree to the following conditions:

1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the Journal under the terms of Creative Commons Attribution License, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this Journal.

2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form of which it has been published by the Journal (for example, to upload the work to the online storage of the Journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this Journal is included.