The metformin influence on proliferation cells of brain tumor in cell culture in vitro


  • Nikolay Lisianyi Romodanov Neurosurgery Institute, Kiev, Ukraine
  • L. Sulenko Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Oleksandr Lisianyi Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Antonina Klyuchnikova Romodanov Neurosurgery Institute, Kiev, Ukraine



метформин, опухоли мозга, глиобластомы, культура клеток in vitrо, эксперимент


Introduction. Today scientists pay much attention to studies of antitumor effect of anti-diabetes medicine metformin on different human tumors, including brain tumor.

The aim of research was to study influence of metformin on glial tumors in cell culture in vitro.

Materials and methods. Antiproliferation and cytotoxical activity of metformin on different brain tumors was studied in cell culture in vitro during 24 h at 37°C with of 0.1% of trypan blue solution. 26 different brain tumors were studied.

Results. Antidiabetes medicine metformin had antiproliferation and cytotoxical activity on cells of different brain tumor in suspension cell culture in vitro. Antitumor effect depended on the medicine dose, large doses —100 mcg/ml were more effective than small ones — 1 mcg/ml. Malignant brain gliomas (glioblastomas) were more sensitive to metformin’s action than astrocytomas of I–II degrees of anaplasia.

Conclusion. The obtained data let us recommend metformin use in neurooncology on stages of combined treatment.

Author Biographies

Nikolay Lisianyi, Romodanov Neurosurgery Institute, Kiev

Neuroimmunology Department

L. Sulenko, Romodanov Neurosurgery Institute, Kiev

Neuroimmunology Department

Oleksandr Lisianyi, Romodanov Neurosurgery Institute, Kiev

Infratentorial Neurooncology Department

Antonina Klyuchnikova, Romodanov Neurosurgery Institute, Kiev

Neuroimmunology Department


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How to Cite

Lisianyi, N., Sulenko, L., Lisianyi, O., & Klyuchnikova, A. (2012). The metformin influence on proliferation cells of brain tumor in cell culture in vitro. Ukrainian Neurosurgical Journal, (2), 28–32.



Original articles