Influence of fetal tissues transplantation on the brain serotoninergic systems in experimental animals after traumatic brain injury
Keywords:severe brain injury, transplantation, fetal nervous tissue, fetal muscular tissue, serotonin, experiment
Introduction. Taking into consideration the high frequency of complications after severe traumatic brain injury (STBI), caused mainly by different brain neuromediator systems integrative disfunctions, a search of methods for their correction is the actual task of neurotraumatology.
Materials and methods. We investigated serotonin and its’ metabolite — 5-hydroxyindolylacetic acid (5-HIAA) levels in structures of brain of experimental animals after STBI, and also influence of fetal nervous (FNT) and fetal muscular (FMT) tissues transplantation on these indexes using high performative method of liquid chromatography.
Results. It was found that serotonin and 5-HIAA levels in the cortex of left (damaged) hemisphere significantly decreased and 30 days after trauma in the hypothalamus of experimental animals serotonin level decreased, but level of 5-HIAA increased. In animals, in which in 2 hours after STBI FNT was transplanted, 5-HIAA level was within a reference level, while serotonin level considerably decreased.
Conclusions. FNT transplantation has the stimulating influence on indolamines’ metabolism, but does not result in complete renewing of serotonin level in some structures of the brain 30 days after STBI. FMT transplantation has strong stimulating influence on the level of serotonin in experimental animals’ brain, testifying to it’s generalized trophic influence on the serotoninergic neurons.
1. Beaumont A, Marmarou A, Czigner A, Yamamoto M, Demetriadou K, Shirotani T, Marmarou C, Dunbar J. The impact-acceleration model of head injury: injury severity predicts motor and cognitive performance after trauma. Neurol Res. 1999 Dec;21(8):742-54. [PubMed]
2. Max JE, Koele SL, Smith WL Jr, Sato Y, Lindgren SD, Robin DA, Arndt S. Psychiatric disorders in children and adolescents after severe traumatic brain injury: a controlled study. J Am Acad Child Adolesc Psychiatry. 1998 Aug;37(8):832-40. [CrossRef] [PubMed]
7. Barkhatova V. Neyrotransmittery I Ekstrapiramidnaya Patologiya [Neurotransmitters And Extrapyramidal Pathology]. Moscow: Meditsina; 1988:176. Russian.
8. Frazer A, Hensler J. Understanding the neuroanatomical organization of serotonergic cells in brain provides insight into the functions of this neurotransmitter. In: Siegel G, Agranoff B, Fisher S, ed. Basic Neurochemistry. 1st ed. Baltimore: Lippincott Williams and Wilkins; 1999:264–268.
9. Gel'fand V, Malamud M, Istratov V. Zakrytaya Cherepno-Mozgovaya Travma [Closed Head Injury]. Chisinau: Shtiintsa; 1986. Rssian.
10. Fedorov V, Bonetskiy A. Opredeleniye katekholaminov plazmy krovi metodom vysokoeffektivnoy zhidkostnoy khromatografii na mikrokolonochnom khromatografe «Millikhrom» [Determination of catecholamines plasma by high performance liquid chromatography, microcolumn chromatograph "Millichrome"]. Laboratornoe delo. 1989;4:21–25. Russian.
11. Nasyrova DI, Urtikova NA, Sapronova AY, Ugryumov MV. Brain is a source of blood serotonin in rats during perinatal development. Russian Journal of Developmental Biology. 2009 Jan;40(1):44–52. [CrossRef] [PubMed]
12. Midzyanovskaya IS, Folomkina AA, Koshtoyants OK, Kuznetsova GD, Bazyan AS. [The Concentration of Serotonin and its Metabolite 5-Hydroxyindolacetic Acid in Brain Structures of Wistar and WAG/Rij Rats and the Influence of Imipramine on Spike-Wave Discharges]. Neurochemical Journal. 2010;27(3):238–244. Russian. [eLIBRARY.ru]
13. Sergiyenko N, Grishchenko V, Loginova. G. Biogennyye Aminy I Vozbudimost' Golovnogo Mozga [Biogenic Amines And Brain Excitability]. Kiev: Nauk. dumka; 1992. Russian.
14. Izzati-zade KF, Basha AV, Demchuk ND. [Serotonin metabolism dysfunction in pathogenesis of nervous system disorders]. Zh Nevrol Psikhiatr Im S S Korsakova. 2004;104(9):62-70. Russian. [PubMed]
15. Otellin VA. [Functional morphology of mediator systems of the brain]. Zh Nevrol Psikhiatr Im S S Korsakova. 1998;98(1):54-8. Russian. [PubMed]
16. Leranth C, Sladek Jr. J, Roth R, Redmond Jr. D. Efferent synaptic connections of dopaminergic neurons grafted into the caudate nucleus of experimentally induced parkinsonian monkeys are different from those of control animals. Experimental Brain Research. 1998;123(3):323-333. [CrossRef] [PubMed]
17. Proshina Y. Funktsional'naya morfologiya gipotalamo-gipofizarno-nadpochechnikovoy sistemy pri tyazheloy cherepno-mozgovoy travme [Functional morphology of the hypothalamic-pituitary-adrenal system with severe traumatic brain injury] [dissertation]. Ryazan (Russia): Ryazan State Medical University; 2007. Russian.
19. Tsymbalyuk V, Vasil'yeva I, Pichkur L. Vliyaniye neyrotransplantatsii na soderzhaniye katekholaminov v strukturakh golovnogo mozga krys posle cherepno-mozgovoy travmy [Neurotransplantation Influence on the content of catecholamines in the brain structures of rats after traumatic brain injury]. Neyrofiziologiya. 1998;30(1):36–41. Russian.
20. Tsymbalyuk V, Vasyl'yeva I, Pariy K. Vyvchennya spetsyfichnosti diyi transplantatu na vidnovlennya vmistu dofaminu u mozku pry eksperymental'niy cherepno-mozkoviy travmi [Study of the specificity of action of the graft to restore the contents of dopamine in the brain in experimental traumatic brain injury]. Visnyk naukovyh doslidzhen. 2006;1:116–119. Russian.
21. Nedelcu J, Klein M, Aguzzi A, Boesiger P, Martin E. Biphasic Edema after Hypoxic-Ischemic Brain Injury in Neonatal Rats Reflects Early Neuronal and Late Glial Damage. Pediatr Res. 1999;46(3):297-304. [CrossRef] [PubMed]
How to Cite
Copyright (c) 2012 Boris Baranenko, Vitaliy Tsymbaliuk, Irina Vasileva, Nataliya Chopik
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ukrainian Neurosurgical Journal abides by the CREATIVE COMMONS copyright rights and permissions for open access journals.
Authors, who are published in this Journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the Journal under the terms of Creative Commons Attribution License, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this Journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form of which it has been published by the Journal (for example, to upload the work to the online storage of the Journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this Journal is included.