Influence of liposome-mediated APOE3 gene transfection of brain tissue on diffuse axonal injury, caused by traumatic brain injury, in experiment


  • Vadym Biloshytsky Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Sergey Mikhalsky D.F.Chebotarev State Institute of Gerontology, Kiev, Ukraine
  • Tatyana Kvitnitskaya-Ryzhova D.F.Chebotarev State Institute of Gerontology, Kiev, Ukraine



traumatic brain injury, gene therapy, apolipoprotein E, diffuse axonal injury


The goal of study was to estimate the structural damage of axons in rats with severe traumatic brain injury (TBI), and the possibilities of such disorders correction by means of gene therapy that launches the synthesis of e3 isoform of apolipoprotein E in brain tissue. Severe diffuse TBI in rats was inflicted under overall anesthesia by free load weighing 450 g, falling from a 1.5 m elevation. The mixture of DOTAP liposome and 25 µg of plasmid vector pCMV•SPORT6 with cDNA of APOE3 gene was infused intraventricularly using ALZET osmotic pumps. Severe experimental TBI is characterized by the damage of hippocampal cytoarchitectonics with destructive-dystrophic changes of all brain elements (neurons, glia, capillaries). The distinguishing feature of this damage is diffuse axonal injury exhibited by pronounced changes of axonal cytoskeleton, axonal edema and deformity, mitochondrial destruction and serious damage to myelin sheathes. The liposome-mediated brain tissue transfection with plasmid vector carrying the gene of e3 isoform of apolipoprotein E has a positive therapeutic effect after TBI including the decrease of diffuse axonal injury.


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How to Cite

Biloshytsky, V., Mikhalsky, S., & Kvitnitskaya-Ryzhova, T. (2011). Influence of liposome-mediated APOE3 gene transfection of brain tissue on diffuse axonal injury, caused by traumatic brain injury, in experiment. Ukrainian Neurosurgical Journal, (3), 43–48.



Original articles