The influence of cultivated autogenous nucleus pulposus cells on structure of intervertebral discs of caudal spine at osteochondrosis model in rats


  • Valeriy Grigorovsky Department of Pathomorphology with Bio-Experimental Unit, Institute of Traumatology and Orthopedics, Kiev, Ukraine
  • Mykhaylo Khyzhnyak Department of Miniinvasive and Laser Spinal Neurosurgery, Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Irina Vasileva Department of Neurobiochemistry, Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Irina Shuba Department of Neurobiochemistry, Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Yuriy Gafiychuk Neurosurgery Department, Military Medical Clinical Center of Southern Region, Odessa, Ukraine



spine, osteochondrosis, asymmetric compression-distension, autogenic cells of intervertebral disc, implantation, histological changes, morphometric indices, experiment


Introduction. The unsolved problem of treatment of intervertebral discs degenerative changes and new cellular technologies development open new possibilities for researches in the different directions, one of which is restoration of structurally-functional properties of the diseased tissues.

Materials and methods. In experiment in rats with model of constant and temporary asymmetric static compression-distension (ASCD) of caudal spine, dynamics of histological changes and morphometric values of intervertebral disc (IVD) tissues were studied, including after implantation of suspension of cultivated autogenous cells of nucleus pulposus.

Results. It was found that autocells implantation has multilateral positive influence on IVD tissues condition: reduces their asymmetry in comparison to the side of compression and distension, frequency of large foci of necrosis in anulus fibrosus (AF).

Conclusions. The parameters, reflecting IVD-tissues reactions on mechanical influence, depend moderately on autocells implantation, at the same time the parameters of IVD tissues condition after ischemic lesion in particular size of foci of AF necrosis on the side of distension and amount of necrotic cells in AF had a tends to optimization in discs with implanted autocells.


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How to Cite

Grigorovsky, V., Khyzhnyak, M., Vasileva, I., Shuba, I., & Gafiychuk, Y. (2013). The influence of cultivated autogenous nucleus pulposus cells on structure of intervertebral discs of caudal spine at osteochondrosis model in rats. Ukrainian Neurosurgical Journal, (2), 28–34.



Original articles