Clinico-morphological and pathogenetic features of glioblastomas, contaminated by herpes simplex virus
Keywords:glioblastoma, apoptosis, Herpes Simplex virus
The purpose. To study clinico-morphological and pathogenetic features of glioblastomas, contaminated by herpes simplex virus (HSV).
Materials and methods. 708 medical records of patients with glioblastoma have been analyzed in the period from 2000 to 2012. Clinical, morphological and pathogrenetic features of tumors flow were studied.
Results. In patients with glioblastomas, contaminated by HSV, we observed rashes in the nasolabial triangle, in 57% — viral DNA in liquor. In glioblastomas, contaminated by HSV, bcl-2 expression in endothelial cells tripled, in tumor cells — doubled.
Conclusions. 1. In some patients with glioblastomas clinical symptoms of herpes infection were observed: rushes on skin, high leucocytes, increased ERS. In liquor of 57% using polymerase chain reaction (CRP) HSV DNA was detected.
2. In glioblastomas tissue HSV antigen was detected, expressed by the tumor and vessel wall and also by vessel wall and tumor tissue at the same time, which is accompanied by infiltration of T-lymphocytes.
3. At contamination of glioblastomas by HSV bcl-2 expression in endothelial cells tripled, in tumor cells — doubled, in all groups in average.
4. Contamination of glioblastomas by HSV has an ambiguous effect on the growth and progression of tumors: in contaminated tumors mitotic activity is reduced, but number and variety of vessels is increased, which is manifested by tumor’s growth acceleration.
Zhukova TV, Nedzved’ MK. Kriterii prognozirovaniya rosta neyroepitelial'nykh opukholey i rekomendatsii po taktike ikh lecheniya [The criteria for neuroepithelial tumors growth predicting and recommendations on tactics of their treatment]. In: Abstract Book of All-Russian Conference “Polenovskie Chteniya”; 2012 April 17-20; Sankt-Peterburg, Russia. Sankt-Peterburg, 2012. p.228. Russian.
Zhukova TV, Nedzved’ MK. Kliniko-morfologicheskiye osobennosti angiogeneza v glioblastomakh, kontaminirovannykh virusom prostogo gerpesa [Clinical and morphological features of angiogenesis in glioblastomas, contaminated with herpes simplex virus]. Med. Zhurn. 2006;2(16):42-43. Russian.
Immonen A, Vapalahti M, Tyynelä K, Hurskainen H, Sandmair A, Vanninen R, Langford G, Murray N, Ylä-Herttuala S. AdvHSV-tk gene therapy with intravenous ganciclovir improves survival in human malignant glioma: a randomised, controlled study. Molecular Therapy. 2004;10(5):967-972. [CrossRef] [PubMed]
Khmara ME, Nedzved’ MK. Patogeneticheskiye mekhanizmy i morfogenez gerpeticheskoy infektsii TSNS i nekotorykh neyroektodermal'nykh opukholey, assotsiirovannykh s virusom prostogo gerpesa [Pathogenetic mechanisms and morphogenesis of herpes infection of CNS and some neuroectodermal tumors associated with herpes simplex virus]. In: Abstract Book of the Congress of neurologists and neurosurgeons of Belarus Republic. Minsk, 2006. p.246. Russian.
Glik B, Pasternak D. Molekulyarnaya biotekhnologiya. Printsipy i primeneniye [Molecular Biotechnology. Principles and Applications]. Moskow: Mir; 2002. Russian.
Patrushev LI. Iskusstvennyye geneticheskiye sistemy [Artificial genetic systems]. Moskva: Nauka; 2005. Russian.
Zhukova TV, Smeyanovich AF, Nedzved’ MK, Bezubik SD, Shirinsky AA, Martynyuk SN, Akhremchuk AI, Khmara MN. Printsipy posleoperatsionnogo lecheniya patsiyentov s neyroepitelial'nymi opukholyami, kontaminirovannymi virusom prostogo gerpesa [Principles of post-operative treatment of patients with neuroepithelial tumors contaminated with herpes simplex virus]. Ros. Neurohirurg. Zhurn. Im. Prof. Polenova. 2013;5:special edition:182.
Kalinin VL. Vvedeniye v molekulyarnuyu virusologiyu [Introduction to molecular virology]. Sankt-Peterburg:SPbGTU; 2002. Russian.
Uglumbekov EG. Gistologiya [Histology]. Moskva: Geotar-med; 2001. Russian.
O’Konek JJ, Boucher PD, Shewach DS. Activation of mismatch repair protects cells against ganciclovir citotoxicity through decreasing errors in DNA replication. Proc. Am. Assoc. Cancer Res. 2007;(48):423-430.
Lins D, Kanakis D, Heinrichs K, Dietzmann CW, Wallesch CW, Mawrin C. Sudden progression of a glioblastoma in partial remission? Clin. Neurol. Neurosurg. 2004;106(4):335-336. [PubMed]
How to Cite
Copyright (c) 2014 Tatiana Zhukova, Arnold Smeyanovich
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ukrainian Neurosurgical Journal abides by the CREATIVE COMMONS copyright rights and permissions for open access journals.
Authors, who are published in this Journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the Journal under the terms of Creative Commons Attribution License, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this Journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form of which it has been published by the Journal (for example, to upload the work to the online storage of the Journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this Journal is included.