Study of mesenchymal stem cells of adipose tissue effect on the growth of experimental glioma of the rats’ brain
DOI:
https://doi.org/10.25305/unj.46594Keywords:
glioma 101.8, mesenchymal stromal cells of adipose tissue, cultivation, experimentAbstract
Introduction. Relevant issue is the study of mesenchymal stem cells (MSC) effect on growth of tumors with different structures, including brain tumors.
Materials and methods. The study was carried out on white rats. Stromal fraction of adult rats adipose tissue (AT) was prepared and cultured and subsequently administered to animals with glioma (strain 101.8).
Results. Culturing cells of AT stromal-vascular adhesive fraction on day 10–12 we observed formation of fibroblast-like cells with MSC phenotype. Triple intraperitoneal injection of AT MSC on day 3, 5 and 8 after experimental glioma cell implantation did not significantly influenced animals longevity. At intracerebral implantation of tumor cells and AT MSC in a ratio 4:1 duration of disease clinical manifestations and illness in rats with glioma increased, that was accompanied by a tendency to mitotic activity reduce in tumor cells in areas between foci of necrosis.
Conclusions. Intracerebral implantation of tumor cells and AT MSC causes prolong disease clinical manifestations and illness in rats with glioma.
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Copyright (c) 2014 Nikolay Lisianyi, Lyudmila Belska, Vera Semenova, Larisa Stayno
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