Phenotypical changes and proliferative potential of mesenchymal stem cells from humans Wharton’s jelly in the cultivation conditions

Authors

  • Vitaliy Tsymbaliuk Restorative Neurosurgery Department, Romodanov Neurosurgery Institute, Kiev, Ukraine https://orcid.org/0000-0001-7544-6603
  • Elena Deriabina Department of Gene Technology, Institute of Genetic and Regenerative Medicine, Kiev, Ukraine
  • Nadezhda Shuvalova Department of Gene Technology, Institute of Genetic and Regenerative Medicine, Kiev, Ukraine
  • Olga Maslova Department of Gene Technology, Institute of Genetic and Regenerative Medicine, Kiev, Ukraine https://orcid.org/0000-0002-9372-896X
  • Ianina Pokholenko Department of Regulatory Mechanisms of Cells, Institute of Molecular Biology and Genetics, Kiev, Ukraine
  • Olena Toporova Department of Regulatory Mechanisms of Cells, Institute of Molecular Biology and Genetics, Kiev, Ukraine
  • Svitlana Shpileva Department of Regulatory Mechanisms of Cells, Institute of Molecular Biology and Genetics, Kiev, Ukraine
  • Vitaliy Kirik Laboratory of Cell and Tissue Cultures of Cell and Tissue Technology Department, Institute of Genetic and Regenerative Medicine, Kiev, Ukraine
  • Leonid Pichkur Restorative Neurosurgery Department, Romodanov Neurosurgery Institute, Kiev, Ukraine https://orcid.org/0000-0003-4716-0960
  • Iuriy Kasianenko Restorative Neurosurgery Department, Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Oleksandr Pichkur Restorative Neurosurgery Department, Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Vitaliy Kordium Department of Regulatory Mechanisms of Cells, Institute of Molecular Biology and Genetics, Kiev, Ukraine

DOI:

https://doi.org/10.25305/unj.45290

Keywords:

mesenchymal stem cells, human umbilical cord, cultivation, proliferative potential

Abstract

Purpose. To develop a protocol for mesenchymal stem cells (MSCs) obtaining from wharton’s jelly of human umbilical cord, to study their phenotypic changes and proliferative potential in culture.

Materials and methods. MSCs from human umbilical cord were cultured for 6 passages. To study morphological features of the cells in a culture we used adapted staining with eosin and gematoksilіn, Romanovsky–Giemsa, and toluidine blue. For nuclear DNA coloring we used intercalators: Hoechst 33342, and DARІ Ethidiumbromid. MSCs surface markers (CD105, CD90, CD73, CD34) expression was studied by FACS-analysis.

Results. In the culture MSCs retain their morphological identity for 2 passages. Further a tendency to MSCs markers expression decreasing was observed, signs of culture degradation, spontaneous chondrogenic and adipogenic differentiation in late passages.

Conclusion. Cultured MSCs of human umbilical cord for 2 passages retain their morphological characteristics and proliferative potential, further they lose multipotent mesenchymal phenotype.

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Published

2015-06-27

How to Cite

Tsymbaliuk, V., Deriabina, E., Shuvalova, N., Maslova, O., Pokholenko, I., Toporova, O., Shpileva, S., Kirik, V., Pichkur, L., Kasianenko, I., Pichkur, O., & Kordium, V. (2015). Phenotypical changes and proliferative potential of mesenchymal stem cells from humans Wharton’s jelly in the cultivation conditions. Ukrainian Neurosurgical Journal, (2), 17–24. https://doi.org/10.25305/unj.45290

Issue

No. 2 (2015)

Section

Original articles

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Copyright (c) 2015 Vitaliy Tsymbaliuk, Elena Deriabina, Nadezhda Shuvalova, Olga Maslova, Ianina Pokholenko, Olena Toporova, Svitlana Shpileva, Vitaliy Kirik, Leonid Pichkur, Iuriy Kasianenko, Oleksandr Pichkur, Vitaliy Kordium

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