In vitro study of proliferation and differentiation cells potention, derived from embryonal human brain
DOI:
https://doi.org/10.25305/unj.130899Keywords:
neural stem cells, neural precursors, differentiation, culturing in vitro, suspension culture, retinol acetate, retinoic acid, vimentin, GFAPAbstract
The proliferation potention and influence of some biomedicines on neuronal differentiation cytostructural peculiarities of the cultivated neural precursors, derived from human cryopreserved neurocells were studied. Proliferying neurocells population was vimentin-positive. The cultivation in vitro of neuronal precursors in DMEM without serum with insulin-transferrin-selenit addition did not lead to their terminal differentiation. Сultivation with retinoic acid stimulated the neuronal-way differentiating cells growth. Some neural precursors differentiated in glyocytes and were GFAP-positive. The obtained results evidence about saved capacity of both neuronal and glyal differentiation ways of cell precursors, derived after long-term cultivation in suspension in DMEM.
References
Гордеева О.Ф., Мануилова Е.С., Гуляева Д.В. и др. Морфологические аспекты дифференцировки эмбриональных стволовых клеток в культуре // Цитология. — 2001. — Т.43, №9. — С.851.
Зозуля Ю.А., Лисяный Н.И., Любич Л.Д. и др. Длительное культивирование in vitro криоконсервированных и нативных нейроклеток эмбрионов человека // Укр. нейрохірург. журн. — 2003. — №2. — С.11–14.
Подгорный О.В., Александрова М.А., Марей М.В. и др. Экспрессия белков — маркеров нейральной дифференцировки в культурах стволовых клеток эмбрионального мозга человека // Цитология. — 2004. — №10. — С.935.
Репин В.С., Ржанинова А.А., Шаменков Д.А. Эмбриональные стволовые клетки: фундаментальная биология и медицина. — М., 2002. — 128 с.
Цимбалюк В.І., Медведєв В.В. Генеалогія, ідентифікація та клінічне використання нейрональних стовбурових прогеніторів // Укр. нейрохірург. журн. — 2003. — №4. — С.8–13.
Аlvarez-Buylla A., Garcia-Verdugo J.M., Tramontin A.D. A unified hypothesis on the lineage of neural stem cells // Nat. Rev. Neurosci. — 2001. — V.2, N4. — P.287–293.
Bain G., Kitchens D., Yao M. et al. ESC express neuronal properties in vitro // Dev. Biol. — 1995. — V.168. — P.342–357.
Barami K., Zhao J., Diaz F.G., Lyman W.D. Comparison of neural precursor cell fate in second trimester human brain and spinal cord // Neurol. Res. — 2000. — V.23, N2–3. —P.260–266.
Bouterfa H., Picht T., Noll E. et al. Retinoids: A promising strategy to inhibit growth and migration of gliomas? // Med. Rediant. Oncol. — 1999. — V.33, N4. — P.437.
Brustle O., Kimberly N., Randal D.L. et al. Embryonic stem cell-derived glial precursors a source of myelinating transplants // Science. — 1999. — V.285. — P.754–756.
Caldwell M.A., He X., Wilkie N. et al. Growth factors regulate the survival and fate of cells derived from human neurospheres // Nat. Biotech. — 2001. — V.19, N5. — P.475–479.
Liu S., Qu Y., Steward T. et al. Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation // Proc. Natl. Acad. Sci. USA. — 2000. — V.97. — P.6126–6131.
Morrison S.J., Shah N.M., Anderson D.J. Regulatory mechanism in stem-like cell biology // Cell. — 1997. — V.88. — P.287–298.
Renoncourt Y., Carroll P., Filippi P. et al. Neurons derived in vitro from ES cells express homeoproteins characteristic of moto- and interneurons // Mech. Dev. — 1998. — V.79. — P.185–187.
Schechter R., Abboud M. Neuronal synthesized insulin roles on neural differentiation within fetal rat neuron cell cultures // Brain Res. Dev. Brain Res. — 2001. — V.127, N1. — P.41–49.
Schuldiner M., Eiges R., Eden A. et al. Induced neuronal differentiation of human embryonic stem cells // Brain. Res. — 2001. — V.913, N2. — P.201–205.
Skogh C., Eriksson C., Kokaia M. et al. Generation of regionally specified neurons in expanded glial cultures derived from the mouse and human lateral ganglionic eminence // Mol. Cel. Neurosci. — 2001. — V.17, N5. — P.811–820.
Stemple D.L., Mahanthappa N.K. Neural stem cells are blasting off // Neuron. — 1997. — V.18. — P.1–4.
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Copyright (c) 2007 V. M. Semenova, L. D. Lyubych, N. I. Lisyany, A. Yu. Petrenko, L. P. Stayno
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