Human glioma proliferative potential investigation in the short-term culture for determination to anablastic chemopreparations sensitivity

Authors

  • I. G. Vasilyeva Romodanov Neurosurgery Institute, Kiev, Ukraine
  • V. D. Rozumenko Romodanov Neurosurgery Institute, Kiev, Ukraine
  • A. Ya. Glavatsky Romodanov Neurosurgery Institute, Kiev, Ukraine
  • N. P. Olexenko Romodanov Neurosurgery Institute, Kiev, Ukraine
  • E. S. Galanta Romodanov Neurosurgery Institute, Kiev, Ukraine
  • N. G. Chopik Romodanov Neurosurgery Institute, Kiev, Ukraine
  • O. I. Tsyubko Romodanov Neurosurgery Institute, Kiev, Ukraine
  • T. N. Vaschulenko Romodanov Neurosurgery Institute, Kiev, Ukraine

DOI:

https://doi.org/10.25305/unj.128594

Keywords:

human glioma, H3-thymidine inclusion, proliferative potential

Abstract

Н3-thymydine accumulation was investigated in the short-term cultures, which were obtained from 20 samples of human brain gliomas. It was confirmed that tumors with accumulation did not differ from the phone level in all histological groups. In other samples the level of labled nucleothyde was very different inside the histological groups. The conditions in wich radioactivity level changed that demonstrated the personal tumor's cells drug sensitivity were discussed.

References

Верхоглядова Т.П., Семенова В.М. Некоторые особенности пролиферативной активности макроглиальных опухолей мозга в культуре тканей // Вопр. нейрохирургии им. Н.Н. Бурденко. — 1982. — №5. — С.36–41.

Зозуля Ю.А., Верхоглядова Т.П., Шамаев М.И., Малышева Т.А. Гистобластические принципы классификации опухолей нервной системы и ее клиническое значение // Укр. нейрохірург. журн. — 2001. — №1. — С.32–41.

Культура животных клеток. Методы / Под. ред. Р. Фрешни. — М.: Мир, 1989. — 302 с.

Руководство по культивированию нервной ткани. Методы. Техника. Проблемы / В.П. Божкова, П.Д. Брежестовский, В.П. Буравлев. и др. — М.: Наука, 1988. — 315 с.

Gordower L., Decaestecker C., Lopes M.B. et al. Determination of growth fraction and cell density to evaluate potential growth of human oligodendroglial and astrocytic tumors // J. Cancer Res. Clin. Oncol. — 1998. — V.124, N8. — P.427–434.

Hoshino T. Cell kinetics of glial tumors // Rev. Neurol. — 1992. — V.148, N6. — P.396–401.

Hoshino T. Prognostic implication of bromdeoxyuridine labeling index of human brain tumors // Cell Tissue Kinet. — 1989. — V.22, N2. —P.125.

Hoshino T., Wilson C.B. Review of basic concepts of cell kinetics as applied to brain tumors // J. Neurosurg. — 1975. —V.42, N2. — P.123–131.

Ito S., Chandler K.L., Prados M.D. et al. Proliferative potential and prognostic evaluation of low-grade astrocytomas // J. Neurooncol. — 1994. — V.19, N1. — P.1–9.

Kunz J., Schmidt A., Janish W. DNA synthesis activity and growth fraction of brain tumors // Acta Histochem. — 1992. — V.42. — P.165–170.

Pertuiset B., Dougherty D., Crameyer C. Stem cell studies of human malignant brain tumors. Part 2: Proliferation kinetics of brain-tumor cells in vitro in early-passage culture // J. Neurosurg. — 1985. — V.63, N3. — P.426–432.

How to Cite

Vasilyeva, I. G., Rozumenko, V. D., Glavatsky, A. Y., Olexenko, N. P., Galanta, E. S., Chopik, N. G., Tsyubko, O. I., & Vaschulenko, T. N. (2005). Human glioma proliferative potential investigation in the short-term culture for determination to anablastic chemopreparations sensitivity. Ukrainian Neurosurgical Journal, (4), 19–25. https://doi.org/10.25305/unj.128594

Issue

Section

Original articles