Влияние липосомальной трансфекции гена аполипопротеина E3 на динамику неврологического и когнитивного дефицита при черепно-мозговой травме в эксперименте

V. V. Biloshytsky; N. Ya. Gridina; L. O. Tsyba; O. M. Velichko

doi:10.25305/unj.108639

Authors

V. V. Biloshytsky Romodanov Neurosurgery Institute, Kiev, Ukraine
N. Ya. Gridina Romodanov Neurosurgery Institute, Kiev, Ukraine
L. O. Tsyba Institute of Molecular Biology and Genetics, NAS of Ukraine, Ukraine
O. M. Velichko Romodanov Neurosurgery Institute, Kiev, Ukraine

DOI:

https://doi.org/10.25305/unj.108639

Keywords:

traumatic brain injury, gene therapy, apolipoprotein E, motor dysfunction, cognitive disorders, experiment

Abstract

The aim of study was to estimate gene therapy influence, that promotes synthesis induction of 3 apolipoprotein E (apoE3) in damaged brain tissue, on motor and memory dysfunction and learning disorders after experimental traumatic brain injury (TBI). Heavy TBI in rats was inflicted under overall anesthesia by free falling from1.5 mof load weighting450 g. The mixture of DOTAP liposome and 25 µg of plasmid vector pCMV•SPORT6 with cDNA of APOE3 gene was infused intraventricularly using ALZET osmotic pump. Posttraumatic neurologic deficits was estimated according to the scale of motor dysfunction at the first day of experiment before TBI infliction and from 1st to 7th day after trauma. Cognitive functions (spatial memory and learning) were tested in Morris water maze during 7–10 days after injury. The obtained results testified that cationic liposome-mediated APOE3 gene transfer caused quicker regress of neurologic and cognitive disorders after experimental TBI.

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