The long-term cultivation in vitro of the cryopreserved and native nerve cells of human embryo
Keywords:
neural stem cells, neural progenitor cells, suspension cultures, retinol acetateAbstract
The long-term cultivation in vitro of neuroprecursors in DMEM without serum lead to the 3–4-fold decrease of the number of cells on the 9-th day of cultivation. Then the number of cells become consistent evidencing about the presence in culture of self-renewing population of stem cells. Retinolacetate stimulate the proliferation of precursors. The method of long-term cultivation of human nerve cells allow to get the enriched population of neuroprecursors with capacity to proliferation in vitro.
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Copyright (c) 2003 Yu. P. Zozulya, N. I. Lisyany, L. D. Lyubych, V. I. Grischenko, A. Yu. Petrenko, G. A. Babiychuk
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