The long-term cultivation in vitro of the cryopreserved and native nerve cells of human embryo

Authors

  • Yu. P. Zozulya Romodanov Neurosurgery Institute, Kiev, Ukraine
  • N. I. Lisyany Romodanov Neurosurgery Institute, Kiev, Ukraine
  • L. D. Lyubych Romodanov Neurosurgery Institute, Kiev, Ukraine
  • V. I. Grischenko Institute of Cryobiology and Cryomedicine, NAS Ukraine, Kharkov, Ukraine
  • A. Yu. Petrenko Institute of Cryobiology and Cryomedicine, NAS Ukraine, Kharkov, Ukraine
  • G. A. Babiychuk Institute of Cryobiology and Cryomedicine, NAS Ukraine, Kharkov, Ukraine

Keywords:

neural stem cells, neural progenitor cells, suspension cultures, retinol acetate

Abstract

The long-term cultivation in vitro of neuroprecursors in DMEM without serum lead to the 3–4-fold decrease of the number of cells on the 9-th day of cultivation. Then the number of cells become consistent evidencing about the presence in culture of self-renewing population of stem cells. Retinolacetate stimulate the proliferation of precursors. The method of long-term cultivation of human nerve cells allow to get the enriched population of neuroprecursors with capacity to proliferation in vitro.

References

Анализ развития стволовых нейральных клеток человека in vitro / Полтавцева Р.А., Марей М.В., Дубровина И.В. и др. // Цитология. — 2001. — Т.43, №9. — С.884–885.

Морфологические аспекты дифференцировки эмбриональных стволовых клеток в культуре / Гордеева О.Ф., Мануилова Е.С., Гуляева Д.В. и др. // Цитология. — 2001. — Т.43, №9. — С.851.

Развитие и дифференцировка мультипотентных нейральных клеток человека in vitro / Полтавцева Р.А., Марей М.В., Дубровина И.В. и др. // Докл. РАН. — 2001.–Т.379, №6. — С.845–849.

Репин В.С., Сухих Г.Т. Медицинская клеточная биология. — М.: БЭБиМ, 1998. — 199 c.

Руководство по культивированию нервной ткани: Методы. Техника. Проблемы // В.П. Божкова, Л.А. Брежетовский, В.М. Буравлев и др. — М.: Наука, 1988. — 318 с.

Сухих Г.Т. Трансплантация фетальных клеток в медицине: настоящее и будущее // Бюл. эксперим. биол. — 1998. — Т.126. Прил.1. — С.3–13.

Эмбриональные стволовые клетки: плюрипотентность, коммитирование, регуляция дифференцировки / Мануилова Е.С., Гордеева О.Ф., Зиновьева Р.Д. и др. // Цитология. — 2001. — Т.43, №9. — С.876.

Barami K., Zhao J., Diaz F.G., Lyman W.D. Cоmparison of neural precursor cell fate in second trimester human brain and spinal cord // Neurol. Res. — 2000. — 23(2–3). — P.260–266.

Caldwell M.A., Нe X., Wilkie N. et al. Growth factors regulate the survival and fate of cells derived from human neurospheres // Nat. Biotech. — 2001. — 19(5). — P.475–479.

Ciccolini F. Identification of two distinct types of multipotent neural precursors that appear sequentially during CNS development // Mol. Cell Neurosci. — 2001. — 17(5). — P.895–907.

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How to Cite

Zozulya, Y. P., Lisyany, N. I., Lyubych, L. D., Grischenko, V. I., Petrenko, A. Y., & Babiychuk, G. A. The long-term cultivation in vitro of the cryopreserved and native nerve cells of human embryo. Ukrainian Neurosurgical Journal, (2), 11–14. Retrieved from https://theunj.org/article/view/107687

Issue

No. 2 (2003)

Section

Original articles

License

Copyright (c) 2003 Yu. P. Zozulya, N. I. Lisyany, L. D. Lyubych, V. I. Grischenko, A. Yu. Petrenko, G. A. Babiychuk

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