DOI: https://doi.org/10.25305/unj.89658

The influence of liposome-mediated APOE3 gene transfection in brain cells on exploratory behavior and emotionality of rats after experimental traumatic brain injury

V. V. Biloshytsky, O. M. Velichko, N. Ya. Gridina, L. O. Tsyba, E. G. Pedachenko

Abstract


The goal of study was to estimate in the experiment the levels of exploratory behavior and emotionality disorders of rats with severe diffuse traumatic brain injury (TBI), and also possibilities of such disorders correction using gene therapy that launches Е3 synthesis in brain tissue — isoform of apolipoprotein E. Severe diffuse TBI in rats was inflicted under overall anesthesia by free load weighting 450 g, falling from 1.5 m height. The mixture of DOTAP liposome and 25 µg of plasmid vector pCMV•SPORT6 with cDNA of APOE3 gene was infused intraventricularly using ALZET osmotic pumps. The exploratory behavior and emotionality of rats in experimental and control groups were studied in open field test 7 days after trauma. The obtained results demonstrated that severe diffuse TBI in rats was characterized by evolution of evident exploratory behavior and emotionality disorders that reflected stress and anxiety increasing. The liposome-mediated brain tissue transfection with plasmid vector carrying the gene of apolipoprotein E Е3 isoform caused regress of posttraumatic exploratory behavior and emotionality disorders and recovery of affected functions.

Keywords


traumatic brain injury; gene therapy; apolipoprotein E; exploratory behavior; emotionality; open field; experiment

References


Experimental traumatic brain injury induces a pervasive hyperanxious phenotype in rats / N.C. Jones, L. Cardamone, J.P. Williams [et al.] // J. Neurotrauma. — 2008. — V.25. — P.1367–1374.

Moore E.L. Mild traumatic brain injury and anxiety sequelae: a review of the literature / E.L. Moore, L. Terryberry-Spohr, D.A. Hope // Brain Injury. — 2006. — V.20. — P.117–132.

Deficits in novelty exploration after controlled cortical impact / A.K. Wagner, B.A. Postal, S.D. Darrah [et al.] // J. Neurotrauma. — 2007. — V.24. — P.1308–1320.

Влияние трансфекции гена аполипопротеина Е человека на структуру гиппокампа и когнитивные нарушения после черепно-мозговой травмы у крыс разного возраста / С.А. Михальский, В.В. Белошицкий, Л.А. Цыба [и др.] // Пробл. старения и долголетия. — 2008. — Т.17, №2. — С.240–258.

Larsson F. Psychological stress and environmental adaptation in enriched vs. impoverished housed rats / F. Larsson, B. Winblad, A.H. Mohammed // Pharmacol. Biochem. Behav. — 2002. — V.73. — P.193–207.

Prenatal stress induces high anxiety and postnatal handling induces low anxiety in adult offspring: correlation with stress-induced corticosterone secretion / M. Vallee, W. Mayo, F. Dellu [et al.] // J. Neurosci. — 1997. — V.17. — P.2626–2636.

Brain-derived neurotrophic factor administration after traumatic brain injury in the rat does not protect against behavioral or histological deficits / G.R. Blaha, R. Raghupathi, K.E. Saatman [et al.] // Neuroscience. — 2000. — V.99. — P.483–493.

Carbonell W.S. Regional and temporal characterization of neuronal, glial, and axonal response after traumatic brain injury in the mouse / W.S. Carbonell, M.S. Grady // Acta Neuropathol. — 1999. — V.98. — P.396–406.

Jorge R.E. Pathophysiologic aspects of major depression following traumatic brain injury / R.E. Jorge, S.E. Starkstein // J. Head Trauma Rehabil. — 2005. — V.20. — P.475–487.

Psychiatric challenges in the first 6 years after traumatic brain injury: cross-sequential analyses of Axis I disorders / T.A. Ashman, L.A. Spielman, M.R. Hibbard [et al.] // Arch. Phys. Med. Rehabil. — 2004. — V.85. — P.36–42.

Magnesium attenuates post-traumatic depression/anxiety following diffuse traumatic brain injury in rats / L. Fromm, B. Pharm, D.L. Heath [et al.] // J. Am. Coll. Nutr. — 2004. — V.23, N5. — P.529–533.

Morton M.V. Psychosocial and emotional sequelae of individuals with traumatic brain injury: a literature review and recommendations / M.V. Morton, P. Wehman // Brain Injury. — 1995. — V.9. — P.81–92.

Depression-like and anxiety-like behavioural aftermaths of impact accelerated traumatic brain injury in rats: A model of comorbid depression and anxiety? / D.K. Pandey, S.K. Yadav, R. Mahesh [et al.] // Behav. Brain Res. — 2009. — V.205, N2. — P.436–442.

Effects of daily versus weekly testing and pre-training on the assessment of neurologic impairment following diffuse traumatic brain injury in rats / C. O’Connor, D.L. Heath, I. Cernak [et al.] // J. Neurotrauma. — 2003. — V.10, N6. — P.985–993.

Magnesium attenuates persistent functional deficits following diffuse traumatic brain injury in rats / R. Vink., C.A. O’Connor, A.J. Nimmo [et al.] // Neurosci. Let. — 2003. — V.336. — P.41–44.

O’Connor C.A. Interaction between anesthesia, gender, and functional outcome task following diffuse traumatic brain injury in rats / C.A. O’Connor, I. Cernak, R. Vink // J. Neurotrauma. — 2003. — V.20, N6. — P.533–541.

Kulkarni S. Open Field Test: its status in psychopharmacology / S. Kulkarni // Ind. J. Pharmacol. — 1977. — V.9. — P.241–246.

Archer J. Tests for emotionality in rats and mice: A review / J. Archer // Animal. Behav. — 1973. — V.21. — P.205–235.

Cyclosporine A affects open field behavior in DA rats / S. Von Horston, M. Exton, J. Voge [et al.] // Pharmacol. Biochem. Behav. — 1998. — V.60. — P.71–76.

Волохова Г. А. Влияние солкосерила на вызванные черепно-мозговой травмой электрографические изменения и поведение крыс / Г.А. Волохова, А.Н. Стоянов // Міжнар. неврол. журн. — 2008. — №2. — С.51–57.

Маркель А.Л. К оценке основных характеристик поведения крыс в тесте “открытого поля” / А.Л. Маркель // Журн. высш. нерв. деятельности им. И.П. Павлова. — 1981. — Т.31, №2. — С.301–307.

Буреш Я. Открытое поле / Я. Буреш, О. Бурешова, Дж. П. Хьюстон; пер. с англ Е.Н. Живописцевой // Методики и основные эксперименты по изучению мозга и поведения. — М.: Высш. шк, 1991. — С.119–121.

Neuroprotective effects of resveratrol against traumatic brain injury in immature rats / U. Sonmez, A. Sonmez, G. Erbil [et al.] // Neurosci. Let. — 2007. — V.420. — P.133–137.

Индивидуально-типологические особенности поведения крыс после легкой механической травмы / Ю.Н. Шанин, В.Н. Цыган, Ж.Г. Александрова, Н.Б. Суворов // Патол. физиология и эксперим. терапия. — 1987. — №1. — С.9–11.

Hughes R. Intrinsic exploration in animals: motives and measurement / R. Hughes // Behav. Processes. — 1997. — V.41. — P.213–226.

Sutcliffe J.S. Influence of gender on working and spatial memory in the novel object recognition task in the rat / J.S. Sutcliffe, K.M. Marshall, J.C. Neill // Behav. Brain. Res. — 2007. — V.177. — P.117–125.

Bramlett H.M. Pathophysiology of cerebral ischemia and brain trauma: similarities and differences / H.M. Bramlett, W.D. Dietrich // J. Cereb. Blood Flow Metab. — 2004. — V.24. — P.133–150.

Leker R.R. Cerebral ischemia and trauma — different etiologies yet similar mechanisms: neuroprotective opportunities / R.R. Leker, E. Shohami // Brain Res. Rev. — 2002. — V.39. — P.55–73.

McIntosh T.K. Neurochemical sequelae of traumatic brain injury / T.K. McIntosh // Cereb. Brain Metab. Rev. — 1994. — V.6. — P.109–162.

Van Reekum R. Can traumatic brain injury cause psychiatric disorders? / R. Van Reekum, T. Cohen, J. Wong // J. Neuropsychiat. Clin. Neurosci. — 2000. — V.12. — P.316–327.

Controlled cortical impact injury affects dopaminergic transmission in the rat striatum / A.K. Wagner, J.E. Sokolovski, D. Ren [et al.] // J. Neurochem. — 2005. — V.95. — P.457–465.

Gender and environmental enrichment impact dopamine transporter expression after experimental traumatic brain injury / A.K. Wagner, X. Chen, A.E. Kline [et al.] // Exp. Neurol. — 2005. — V.195. — P.475–483.

Novelty seeking and stereotypic activation on behavior in mice with disruption of the Dat1 gene / V.M. Pogorelov, R.M. Rodriguiz, M.L.. Insco [et al.] // Neuropsychopharmacology. — 2005. — V.30. — P.1818–1831.

Regional levels of lactate and norepinephrine after experimental brain injury / M.R. Prasad, C. Ramaiah, T.K. McIntosh [et al.] // J. Neurochem. — 1994. — V.63. — P.1086–1094.

Boyeson M.G. Intraventricular norepinephrine facilitates motor recovery following sensorimotor cortex injury / M.G. Boyeson, D.M. Feeney // Pharmacol. Biochem. Behav. — 1990. — N5. — P.497–501.

Elevated cholecystokininergic tone constitutes an important molecular/neuronal mechanism for the expression of anxiety in the mouse / Q. Chen, A. Nakajima, C. Meachem [et al.] // Proc. Natl. Acad. Sci. USA. — 2006. — V.103. — P.3881–3886.

Increase of insulin-like growth factor (IGF)-1, IGF binding protein-2, and -4 mRNAs following cerebral contusion / A.-C.S. Nordqvist, H. von Holst, S. Holmin [et al.] // Mol. Brain Res. — 1996. — V.38. — P.285–293.

The etiology of poststroke depression: a review of the literature and a new hypothesis involving inflammatory cytokines / G. Spalletta, P. Bossu, A. Ciaramella [et al.] // Mol. Psychiatry. — 2006. — V.11. — P.984–991.

Влияние липосомальной трансфекции гена аполипопротеина Е3 на динамику неврологического и когнитивного дефицита при черепно-мозговой травме в эксперименте / В.В. Белошицкий, Н.Я. Гридина, Л.А. Цыба [и др.] // Укр. нейрохірург. журн. — 2009. — №2. — С.59–63.


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