The model of lateral spinal cord hemisection. Part I. The technical, pathomorphological, clinical and experimental peculiarities

Authors

  • Vitaliy Tsymbaliuk Romodanov Neurosurgery Institute, Ukraine https://orcid.org/0000-0001-7544-6603
  • Volodymyr Medvediev Bogomolets National Medical University, Ministry of Healthcare of Ukraine, Kiev, Ukraine
  • Vera Semenova Romodanov Neurosurgery Institute, Ukraine
  • Nina Grydina Romodanov Neurosurgery Institute, Ukraine
  • Yuriy Senchyk Kyiv City Clinical Emergency Hospital, Kyiv, Ukraine
  • Olga Velychko Romodanov Neurosurgery Institute, Ukraine
  • Sergiy Dychko Romodanov Neurosurgery Institute, Ukraine
  • Victoria Vaslovych Romodanov Neurosurgery Institute, Ukraine https://orcid.org/0000-0001-8574-6407

DOI:

https://doi.org/10.25305/unj.72605

Keywords:

model of spinal cord injury, left-side lateral spinal cord hemisection, experiment

Abstract

Relevance of the topic. A promising tool for restoring the spinal cord function is the application of tissue neuroengineering. The approbation of its techniques is possible on qualitative spinal cord injury models that makes the transplantation of solid neuroengineered matrices feasible.

Objective. To optimize and test a model of lower thoracic rat’s spinal cord hemisection.

Materials and methods. 2 groups of experimental animals: 1 — mature animals (3–6 months, n=40) and 2 — young animals (1 month, n=32), the injury — left-side spinal cord hemisection (LHS) at Т11 level; monitoring of hindlimbs’ function (BBB scale), pathomorphological study.

Results. LHS provides simultaneous display of a slight, moderate and severe spinal cord injury (the correlates, respectively, are: the function deficit of a contralateral hindlimb [CH] and an ipsilateral hindlimb [IH] among the animals with better and worse recovery indicators). As of the 11th week of the observation a function indicator was 3,2±0,58 points under BBB scale and 5,31±0,79 (p<0.05) in group 2, which indicates a significant fullness of the intersection of all descending fibers. Mortality in group 1 at the stage of intervention and in the acute phase of trauma consists 25%, in the remote phase — 15%, including 5 percent of animals with bilateral injury. At full bilateral intersection mortality within 10 days with relevant conditions of detention comprises 100 percent.

Conclusion. LHS model is technically simple, easy to be reproduced, has low mortality under the condition of deep ipsilateral spinal cord function deficit, provides for three simultaneous options of spinal cord injury, is adapted for testing the neuroengineering tools.

Author Biographies

Vitaliy Tsymbaliuk, Romodanov Neurosurgery Institute

Restorative Neurosurgery Department

Volodymyr Medvediev, Bogomolets National Medical University, Ministry of Healthcare of Ukraine, Kiev

Department of Neurosurgery

Vera Semenova, Romodanov Neurosurgery Institute

Tissue Cultivating Laboratory

Nina Grydina, Romodanov Neurosurgery Institute

Laboratory of Experimental Neurosurgery

Yuriy Senchyk, Kyiv City Clinical Emergency Hospital, Kyiv

Department of Spinal and Vertebral Surgery

Olga Velychko, Romodanov Neurosurgery Institute

Laboratory of Experimental Neurosurgery

Sergiy Dychko, Romodanov Neurosurgery Institute

1st Department of Anesthesiology and Intensive Care

Victoria Vaslovych, Romodanov Neurosurgery Institute

Electronic Microscopy Laboratory

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Published

2016-06-26

How to Cite

Tsymbaliuk, V., Medvediev, V., Semenova, V., Grydina, N., Senchyk, Y., Velychko, O., Dychko, S., & Vaslovych, V. (2016). The model of lateral spinal cord hemisection. Part I. The technical, pathomorphological, clinical and experimental peculiarities. Ukrainian Neurosurgical Journal, (2), 18–27. https://doi.org/10.25305/unj.72605

Issue

Section

Original articles