DOI: https://doi.org/10.25305/unj.54146

Titer of antibodies to cytomegalovirus in blood of patients at brain tumors

Nikolay Lisianyi, Tatyana Malysheva, Oleksandr Lisianyi, Antonina Klyuchnikova, Larisa Kot, Diana Stanetskaya, D. Besruchko

Abstract


The role of cytomegalovirus (CMV), induction and stimulation of malignant tumors growth of different localization, including brain tumors, is intensively studied in the recent years. Data about this virus importance in brain tumors formation are contradictory. The aim of the research was to study titers of antibodies to CMV and herpes simplex virus (HSV) in the blood of neurooncological patients and presence of virus in brain tumors.

Materials and methods. Blood serum was studied in 66 patients with different brain tumors, in 50 — with chronic inflammatory diseases of the nervous system (first control group), in 50 — with chronic gynecopathies (second control group), and also 42 cytologic imprint of tumors, been removed at operation, were studied. Antibodies to the viruses were determined using accepted methods of enzyme immunoassay, the virus in imprints was determined using kits «CMV monoskan».

Results. It was found that antibodies to HSV were determined frequently at neurological diseases and gynecopathology than at neurooncology. Antibodies to CMV, unlike HSV, were determined more frequently at neurooncology, theit titer was 2–4 times higher than at other diseases. In the cells of malignant brain gliomas (glioblastomas, medulloblastomas) CMV antigens were determined frequently than in bening tumors that indicated high tropism of CMV to malignant tumors and perhaps it’s stimulating influence on the oncogenesis.

Conclusions. CMV-infection more frequently than HSV accompanies brain tumors growth.

Keywords


cytomegalovirus (CMV); antibody; brain tumors; herpes simplex virus (HSV)

References


1. Harkins L, Matlaf L, Soroceanu L Klemm K, Britt WJ, Wang W, Bland KI, Cobbs CS. Detection of human cytomegalovirus in normal and neoplastic breast epithelium. Herpesviridae. 2010;1(1):8-16. [CrossRef] [PubMed]

2. Soroceanu L, Cobbs C. Is HCMV a tumor promoter?. Virus Research. 2011;157(2):193-203. [CrossRef] [PubMed]

3. Melnick M, Sedghizadeh P, Allen C, Jaskoll T. Human cytomegalovirus and mucoepidermoid carcinoma of salivary glands: Cell-specific localization of active viral and oncogenic signaling proteins is confirmatory of a causal relationship. Exp Mol Pathol. 2012;92(1):118-25. [CrossRef] [PubMed]

4. Cobbs CS, Harkins L, Samanta M, Gillespie GY, Bharara S, King PH, Nabors LB, Cobbs CG, Britt WJ. Human cytomegalovirus infection and expression in human malignant glioma. Cancer Res. 2002 Jun 15;62(12):3347-50. [PubMed]

5. Scheurer M, Bondy M, Aldape K, Albrecht T, El-Zein R. Detection of human cytomegalovirus in different histological types of gliomas. Acta Neuropathol. 2008;116(1):79-86. [CrossRef] [PubMed]

6. Moore P, Chang Y. Why do viruses cause cancer? Highlights of the first century of human tumour virology. Nature Reviews Cancer. 2010;10(12):878-89. [CrossRef] [PubMed]

7. Shen Y, Zhu H, Shenk T. Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins. Proc Natl Acad Sci. USA. 1997;94(7):3341-5. [CrossRef] [PubMed]

8. Cinatl J, Vogel J, Kotchetkov R, Wilhelm Doerr H. Oncomodulatory signals by regulatory proteins encoded by human cytomegalovirus: a novel role for viral infection in tumor progression. FEMS Microbiol Rev. 2004;28(1):59-77. [CrossRef] [PubMed]

9. Poltermann S, Schlehofer B, Steindorf K, Schnitzler P, Geletneky K, Schlehofer J. Lack of association of herpesviruses with brain tumors. J Neurovirol. 2006;12(2):90-9. [CrossRef] [PubMed]

10. Lau S, Chen Y, Chen W, Diamond DJ, Mamelak AN, Zaia JA, Weiss LM. Lack of association of cytomegalovirus with human brain tumors. Mod Pathol. 2004;18(6):838-43. [CrossRef] [PubMed]

11. Ranganathan P, Clark P, Kuo J, Salamat M, Kalejta R. Significant Association of Multiple Human Cytomegalovirus Genomic Loci with Glioblastoma Multiforme Samples. J Virol. 2011;86(2):854-64. [CrossRef]

12. Rahbar A, Stragliotto G, Orrego A, Peredo I, Taher C, Willems J, Söderberg-Naucler C. Low levels of human cytomegalovirus infection in glioblastoma multiforme associates with patient survival; -a case-control study. Herpesviridae. 2012 Mar 16;3:3. [CrossRef] [PubMed]

13. Baryawno N, Rahbar A, Wolmer-Solberg N, Taher C, Odeberg J, Darabi A, Khan Z, Sveinbjörnsson B, FuskevÅg OM, Segerström L, Nordenskjöld M, Siesjö P, Kogner P, Johnsen JI, Söderberg-Nauclér C. Detection of human cytomegalovirus in medulloblastomas reveals a potential therapeutic target. J Clin Invest. 2011;121(10):4043-55. [CrossRef] [PubMed]

14. Lisyanyy AN, Malysheva TA, Stanetskaya DN. Immunofluorestsentnoye opredeleniye TSMV antigenov v opukholyakh golovnogo mozga [Immunofluorescence determination of CMV antigens in brain tumors]. Ímunologíya ta alergologíya. Teoríya ta praktika. 2012;2:39. Russian.

15. Kachkov IA, Biktimirov RG, Zakharov AV, Chumakov VA, Suchkov SV. Glial'nyye opukholi golovnogo mozga:klassifikatsiya, immunopatogenez i immuno genodiagnostika [Glial brain tumors: classification, Immunopathogenesis and immuno gene diagnostics]. Vestn. RAMN. 2005;6:36-42. Russian.

16. Johnsen J, Baryawno N, Söderberg-Nauclér C. Is human cytomegalovirus a target in cancer therapy? Oncotarget. 2011;2(12):1329-38. [CrossRef] [PubMed]


GOST Style Citations


1. Detection of human cytomegalovirus in normal and neoplastic breast epithelium / L.E. Harkins, L.A. Matlaf, L. Soroceanu, K. Klemm, W.J. Britt, W. Wang, K.I. Bland, C.S. Cobbs // Herpesviridae. — 2010. — N1. — P.8–16.

2. Soroceanu L. Is HCMV a tumor promoter? / L. Soroceanu, C.S. Cobbs // Virus Res. — 2011. — V.157. — P.193–203.

3. Melnick M. Human cytomegalovirus and mucoepidermoid carcinoma of salivary glands: Cell-specific localization of active viral and oncogenic signaling proteins is confirmatory of a causal relationship / M. Melnick, P.P. Sedghizadeh, C.N. Allen // Exp. Mol. Pathol. — 2012. — V.92. — P.118–125.

4. Human cytomegalovirus infection and expression in human malignant glioma / C. Cobbs, L. Harkins, M. Samanta, G.Y. Gillespie, S. Bharara, P.H. King, L.B. Nabors, C.G. Cobbs, W.J. Britt // Cancer. — 2002. — V.62. — P.3347–3350.

5. Detection of human cytomegalovirus in different histological types of gliomas / M.E. Scheurer, M.L. Bondy, K.D. Aldape, T. Albrecht, R. El-Zein // Acta Neuropathol. — 2008. — V.116. — P.79–86.

6. Moore P.S. Why do viruses cause cancer? Highlights of the first century of human tumour virology / P.S. Moore, Y. Chang // Nat. Rev. Cancer. — 2010. — V.10. — P.878–889.

7. Shen Y. Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate «hit-and-run» oncogenic transformation in cooperation with the adenovirus E1A proteins / Y. Shen, H. Zhu, T. Shenk // Proc. Natl. Acad. Sci. USA. — 1997. — V.94, N7. — P.3341–3345.

8. Oncomodulatory signals by regulatory proteins encoded by human cytomegalovirus: a novel role for viral infection in tumor progression / J. Cinatl Jr., J.U. Vogel, R. Kotchetkov, D.H. Wilhelm // FEMS Microbiol. Rev. — 2004. — V.28, N1. — P.59–77.

9. Lack of association of herpesviruses with brain tumors / S. Poltermann, B. Schlehofer, K. Steindorf, P. Schnitzler, K. Geletneky, J.R. Schlehofer // J. Neurovirol. — 2006. — V.12, N2. — P.90–99.

10. Lack of association of cytomegalovirus with human brain tumors / S. Lau, Y.Y. Chen, W.G. Chen, D.J. Diamond, A.N. Mamelak, J.A. Zaia, L.M. Weiss // Mod. Pathol. — 2005. — V.18, N6. — P.838–843.

11. Significant association of multiple human cytomegalovirus genomic loci with glioblastoma multiforme samples / P.A. Ranganathan, P. Clark, J. Kuo, M.S. Salamat, R.F. Kalejta // J. Virol. — 2012. — V.86, N2. — P.854–864.

12. Low levels of human cytomegalovirus infection in glioblastoma multiforme associates with patient survival; a case-control study / A. Rahbar, G. Stragliotto, A. Orrego, I. Peredo, C. Taher, J. Willems, C. Sцderberg-Naucler // Herpesviridae. — 2012. — V.3, N3. — P. Published online 2012 March 16. doi: 10.1186/2042-4280-3-3PMCID.

13. Detection of human cytomegalovirus in medulloblastomas reveals a potential therapeutic target / N. Baryawno, A. Rahbar, N. Wolmer-Solberg, C. Taher, J. Odeberg, A. Darabi, Z. Khan, B. Sveinbjцrnsson, O.M. FuskevЕg, L. Segerstrцm, M. Nordenskjцld, P. Siesjц, P. Kogner, J.I. Johnsen, C. Sцderberg-Nauclйr // J. Clin. Invest. — 2011. — V.121. — P.4043–4055.

14. Лисяный А.Н. Иммунофлуоресцентное определение ЦМВ антигенов в опухолях головного мозга // А.Н. Лисяный, Т.А. Малышева, Д.Н. Станецкая // Імунологія та алергологія. Теорія та практика. — 2012. — Додаток №2. — С.39.

15. Глиальные опухоли головного мозга:классификация, иммунопатогенез и иммуногенодиагностика / И.А. Качков, Р.Г. Биктимиров., А.В. Захаров, В.А. Чумаков, С.В. Сучков // Вестн. РАМН. — 2005. — №6. — С.36–42.

16. Johnsen J.I. Is human cytomegalovirus a target in cancer therapy? / J.I. Johnsen, N. Baryawno,S. Wolmer-Solberg// Oncotarget. — 2011. — V.2. — P.1329–1338.

 





Copyright (c) 2013 Nikolay Lisianyi, Tatyana Malyshevа, Oleksandr Lisianyi, Antonina Klyuchnikova, Larisa Kot, Diana Stanetskaya, D. Besruchko

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.