Study of mesenchymal stem cells of adipose tissue effect on the growth of experimental glioma of the rats’ brain

Authors

  • Nikolay Lisianyi Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Lyudmila Belska Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Vera Semenova Romodanov Neurosurgery Institute, Kiev, Ukraine
  • Larisa Stayno Romodanov Neurosurgery Institute, Kiev, Ukraine

DOI:

https://doi.org/10.25305/unj.46594

Keywords:

glioma 101.8, mesenchymal stromal cells of adipose tissue, cultivation, experiment

Abstract

Introduction. Relevant issue is the study of mesenchymal stem cells (MSC) effect on growth of tumors with different structures, including brain tumors.

Materials and methods. The study was carried out on white rats. Stromal fraction of adult rats adipose tissue (AT) was prepared and cultured and subsequently administered to animals with glioma (strain 101.8).

Results. Culturing cells of AT stromal-vascular adhesive fraction on day 10–12 we observed formation of fibroblast-like cells with MSC phenotype. Triple intraperitoneal injection of AT MSC on day 3, 5 and 8 after experimental glioma cell implantation did not significantly influenced animals longevity. At intracerebral implantation of tumor cells and AT MSC in a ratio 4:1 duration of disease clinical manifestations and illness in rats with glioma increased, that was accompanied by a tendency to mitotic activity reduce in tumor cells in areas between foci of necrosis.

Conclusions. Intracerebral implantation of tumor cells and AT MSC causes prolong disease clinical manifestations and illness in rats with glioma.

Author Biographies

Nikolay Lisianyi, Romodanov Neurosurgery Institute, Kiev

Department of Neuroimmunology

Lyudmila Belska, Romodanov Neurosurgery Institute, Kiev

Neuroimmunology Department

Vera Semenova, Romodanov Neurosurgery Institute, Kiev

Laboratory of Tissues Cultivation

Larisa Stayno, Romodanov Neurosurgery Institute, Kiev

Laboratory of Tissues Cultivation

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Published

2014-12-24

How to Cite

Lisianyi, N., Belska, L., Semenova, V., & Stayno, L. (2014). Study of mesenchymal stem cells of adipose tissue effect on the growth of experimental glioma of the rats’ brain. Ukrainian Neurosurgical Journal, (4), 11–16. https://doi.org/10.25305/unj.46594

Issue

Section

Original articles