DOI: https://doi.org/10.25305/unj.45289

Lectins use for receive and study of glioma C6 cells with stem cells properties

Nikolay Lisianyi, Irina Gnedkova, Diana Stanetskaya, Volodymyr Rozumenko, Alexandr Glavatskiy, Anna Shmeleva, Tatyana Malysheva, Oksana Chernenko, Mаrianna Gnedkova

Abstract


The purpose — to enrich suspension of glioma C6 cells in rats with stem tumor stem cells using lectins sorbed on plastic.

Materials and methods. Lectins of beans (Phaseolus vulgaris — PHA), concanavalin (ConA), peanuts (peanut agglutinin — PNA), mistletoe (Mil), labrum anagyroides agglutinin (LABA), soybean (SBA) were used to separate glioma C6 cells.

Glioma C6 cells were cultivated with sorbed lectins for 24 h and were injected into the brain of the rats.

Results. Tumorogenic activity was significantly higher in fractions of cells adhered to plastic with adsorbed lectins PHA, ConA, PNA, LABA and SBA, that was an indirect evidence of these fractions enriched by tumor stem cells. Lectin Mil exert an antitumor effect that contributed to rats’ lifetime increasing.

Conclusions. The results of the study indicate the possibility of tumor cells division using lectins.


Keywords


глиома С6; стволовые клетки опухоли; лектины

References


Brescia P, Richichi C, Pelicci G. Current Strategies for Identification of Glioma Stem Cells: Adequate or Unsatisfactory? Journal of Oncology. 2012;2012:1-10. CrossRef

Dey M, Ulasov I, Tyler M, Sonabend A, Lesniak M. Cancer Stem Cells: The Final Frontier for Glioma Virotherapy. Stem Cell Reviews and Reports. 2010;7(1):119-129. CrossRef

Dodla M, Young A, Venable A et al. Differing Lectin Binding Profiles among Human Embryonic Stem Cells and Derivatives Aid in the Isolation of Neural Progenitor Cells. PLoS ONE. 2011;6(8):e23266. CrossRef

Christensen K, Schrøder H, Kristensen B. CD133 identifies perivascular niches in grade II–IV astrocytomas. Journal of Neuro-Oncology. 2008;90(2):157-170. CrossRef

Schraen-Maschke S. Role of oligomannosidic N-glycans in the proliferation, adhesion and signalling of C6 glioblastoma cells. Biochimie. 2003;85(1-2):219-229. CrossRef

Angelastro J, Lame M. Overexpression of CD133 Promotes Drug Resistance in C6 Glioma Cells. Molecular Cancer Research. 2010;8(8):1105-1115. CrossRef

Tucker-Burden C, Chappa P, Krishnamoorthy M et al. Lectins Identify Glycan Biomarkers on Glioblastoma-Derived Cancer Stem Cells. Stem Cells and Development. 2012;21(13):2374-2386. CrossRef

Dai L, Liu Y, He J et al. Differential profiling studies of N-linked glycoproteins in glioblastoma cancer stem cells upon treatment with γ-secretase inhibitor. PROTEOMICS. 2011;11(20):4021-4028. CrossRef

Chen R, Nishimura M, Bumbaca S et al. A Hierarchy of Self-Renewing Tumor-Initiating Cell Types in Glioblastoma. Cancer Cell. 2010;17(4):362-375. CrossRef

Wang Y, Nakagawa M, Garitaonandia I et al. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis. Cell Res. 2011;21(11):1551-1563. CrossRef

Son M, Woolard K, Nam D, Lee J, Fine H. SSEA-1 Is an Enrichment Marker for Tumor-Initiating Cells in Human Glioblastoma. Cell Stem Cell. 2009;4(5):440-452. CrossRef

Venable A, Mitalipova M, Lyons I, Jones K, Shin S, Pierce M, Stice S. Lectin binding profiles of SSEA-4 enriched, pluripotent human embryonic stem cell surfaces. BMC Dev Biol. 2005;5(1):15. CrossRef

Lou Y, Wang P, Yeh S et al. Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers. Proceedings of the National Academy of Sciences. 2014;111(7):2482-2487. CrossRef

Butakov A, Oganezov V, Pinegin P. Spektrofotometricheskoye opredeleniye adgezivnoy sposobnosti polimorfoyadernykh leykotsitov perifericheskoy krovi. Immunologiya. 2015;5:71–73. Russian.

Antonyuk V. Lektyny ta yikh syrovynni dzherela. Lviv: Kvart; 2005:554. Ukrainian.

Nduom E, Hadjipanayis C, Van Meir E. Glioblastoma Cancer Stem-Like Cells: Implications for Pathogenesis and Treatment. The Cancer Journal. 2012;18(1):100-106. CrossRef


GOST Style Citations


Brescia P. Current strategies for identification of glioma stem cells: Adequate or unsatisfactory? / P. Brescia, Ch. Richichi, G. Pehcci // J. Oncol. — 2012. — e376894.

Cancer stem cells: the final frontier for glioma virotherapy/ M. Dey, I.V. Ulasov, M.A. Tyler, A.M. Sonabend, M.S. Lesniak // Stem. Cell Rev. — 2011. — V.7, N1. — P.119–129.

Differening lectin binding prolfiles among human embryonic stem cells and derivates aid in the isolation of neural progeny tor cells /M.C. Dodla, A. Young., A.I. Venable, K. Hasneen, R.R. Rao, D.W. Machacek, S.L. Stice // Plos One. — 2011. — V.6, N8. — e23266.

Christensen K. CD133 identifies perivascular niches in grade II–IV astrocytomas / K. Christensen, H.D. Schroder, B.W. Kristensen // J. Neurooncol. — 2008. — V.90, N2. — P.157–170.

Schraen-Maschke S. Role of oligomannosidic N-glycans in the proliferation, adhesion and signaling of C6 glioblastoma cells / S. Schraen-Maschke, J.P. Zanetta // Biochimie. — 2003. — V85, N1–2. — P.219–229.

Angelastro J.M. Overeexpression of CD133 promotes drug resistance in C6 glioma cells / J.M. Angelastro, M.W. Lame // Mol. Cancer Res. — 2010. — V.8, N8. — P.1105–1115.

Lectins identify glycan biomarkers on glioblastoma-derived cancer stem cells / C. Tucker-Burden, P. Chappa, M. Krishnamoonthy, B.A. Gerwe, C.D. Scharer, J. Heimburg-Molinaro, W. Harris, S.N. Usta, C.D. Eilertson, C.G. Hadjipanayis, S.L. Stice, D.J. Brat, R.J. Nash // Stem Cells Dev. — 2012. — V.21, N13. — P.2374–2386.

Differential profiling studies of N-linked glycoproteins in glioblastoma cancer stem cells upon treatment with γ-secretase ingibitor / L. Dai., Y. Liu, J. He, C.G. Flack, C.E. Talsma, J.G. Crowley, K.M. Muraszko, X. Fan, D.M. Lubman // Proteomics. — 2011. — V11, N20. — P.4021–4028.

Chen R. A hierarchy of self-renewing tumor-initiating cell types in glioblastoma / R. Chen, M.C. Nishimura, S.M. Bumbaca // Cancer Cell. — 2010. — V.17, N4. — P362–375.

Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis / J.Ch. Wang, M. Nakagawa I. Garitaonandia, I. Slavin, G. Altun, R.M. Lacharite, K.L. Nazor, H.T. Tran, C.L. Lynch, T.R. Leonardo, Y. Liu, S.E. Peterson, L.C. Laurent, S. Yamanaka, J.F. Loring // Cell Res. — 2011. — V.21, N11. — P.1551–1563.

SSEA-1 is an enrichment marker for tumor-initiating cells in human glioblastoma / M.J. Son, K. Woolard, D.H. Nam, J. Lee, H.A. Fine // Cell Stem Cell. — 2009. — V.4, N5. — P.440–452.

Lectin binding profiles of SSEA4 enriched pluripotent human embryonic stem cell surfaces / A. Venable, M. Metalipova, I. Lyons, K. Jones, S. Shin, M. Pierce, S. Stice // BMC. Dev. Biol. — 2005. — V.5. — P.15.

Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers / Y.W. Lou, P.-Y. Wang, S.Ch. Yeh, P.K. Chuang, S.T, C.Y. Wu, K.H. Khoo, M. Hsiao, T.L. Hsu, C.H. Wong // Proc. Natl. Acad. Sci. USA. — 2014. — V.111, N7. — P.2482–2487.

Бутаков А.А. Спектрофотометрическое определение адгезивной способности полиморфоядерных лейкоцитов периферической крови / А.А. Бутаков, В.Г. Оганезов, Б.В. Пинегин [и др.] // Иммунология. — 1991 — №5. — С.71–73.

Антонюк В.О. Лектини та їх сировинні джерела / В.О. Антонюк/ — Львів: Кварт, 2005. — 554 с.

Nduom E.R.T., Glioblastoma cancer stem like cells — implications for pathogenesis and treatment / E.R.T. Nduom, C.G. Hadjipanavis, E.G. Meir // Cancer J. — 2012. — V.18, N1. — P.100–106.





Copyright (c) 2015 Nikolay Lisianyi, Irina Gnedkova, Diana Stanetskaya, Volodymyr Rozumenko, Alexandr Glavatskiy, Anna Shmeleva, Tatyana Malysheva, Oksana Chernenko, Mаrianna Gnedkova

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.