The neuronal stem cells effect on inner ear structure in experimental gentamicin ototoxicity

Irina I. Sapizhak, Hryhoriy E. Timen, Vira M. Semenova, Lyudmyla P. Stayno


Objective. To examine the effectiveness of suspensions of embryonic stem cells in experimentally induced aminoglycoside ototoxicity in Guinea pigs.

Materials and methods. The study involved 35 adult Guinea pigs. The hearing loss was caused by administration of gentamicin 100 mg per KBW. Intratympanal suspension of 2 million neuronal steam cells in 0.5 mg and sub occipital suspension of 2 million cells in 0.5 mg were administered on the 1st and 15th day.

Results. The methodology of using and preparation of the suspension from neuronal stem cells enables to provide their necessary number for transplantation in vivo, remaining the cell characteristics.

Aminoglycoside ototoxity caused by a 14-day administration of gentamicin to Guinea pigs in a dose of 100 mg/KBW was accompanied by general intoxication: reduced appetite, weight loss, frequent urination, hair falling out. The administration of neuronal stem cells on the first day, before and after aminoglycoside administration, completely, and on 15 partially neutralizes the symptoms.

Fourteen-day administration of gentamicin to Guinea pigs causes a violation of the architectonics and morphology of inner ear microcirculation in psalterial cords.

NSC administration on the first day of simulated aminoglycoside ototoxicity prevented its development if 14-day injections of gentamycin, that is confirmed by ABR and morphologically.

Conclusions. The conducted experiments show the feasibility of the chosen direction to explore the potential use of neuronal stem cells for the prevention of aminoglycoside ototoxic effects in Guinea pigs.


sensoneural hearing loss; amynoglycoside antibiotic; Guinea pigs; neuronal steam cells; morphological changes


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