The neuronal stem cells effect on inner ear structure in experimental gentamicin ototoxicity
DOI:
https://doi.org/10.25305/unj.115236Keywords:
sensoneural hearing loss, amynoglycoside antibiotic, Guinea pigs, neuronal steam cells, morphological changesAbstract
Objective. To examine the effectiveness of suspensions of embryonic stem cells in experimentally induced aminoglycoside ototoxicity in Guinea pigs.
Materials and methods. The study involved 35 adult Guinea pigs. The hearing loss was caused by administration of gentamicin 100 mg per KBW. Intratympanal suspension of 2 million neuronal steam cells in 0.5 mg and sub occipital suspension of 2 million cells in 0.5 mg were administered on the 1st and 15th day.
Results. The methodology of using and preparation of the suspension from neuronal stem cells enables to provide their necessary number for transplantation in vivo, remaining the cell characteristics.
Aminoglycoside ototoxity caused by a 14-day administration of gentamicin to Guinea pigs in a dose of 100 mg/KBW was accompanied by general intoxication: reduced appetite, weight loss, frequent urination, hair falling out. The administration of neuronal stem cells on the first day, before and after aminoglycoside administration, completely, and on 15 partially neutralizes the symptoms.
Fourteen-day administration of gentamicin to Guinea pigs causes a violation of the architectonics and morphology of inner ear microcirculation in psalterial cords.
NSC administration on the first day of simulated aminoglycoside ototoxicity prevented its development if 14-day injections of gentamycin, that is confirmed by ABR and morphologically.
Conclusions. The conducted experiments show the feasibility of the chosen direction to explore the potential use of neuronal stem cells for the prevention of aminoglycoside ototoxic effects in Guinea pigs.
References
1. Kersigo J, Fritzsch B. Inner ear hair cells deteriorate in mice engineered to have no or diminished innervation. Front Aging Neurosci. 2015 Mar 18;7:33. [CrossRef] [PubMed] [PubMed Central]
2. Huisman MA, Rivolta MN. Neural crest stem cells and their potential application in a therapy for deafness. Front Biosci (Schol Ed). 2012 Jan 1;4:121-32. Review. [CrossRef] [PubMed]
3. McKay R. Stem cells in the central nervous system. Science. 1997 Apr 4;276(5309):66-71. Review. [CrossRef] [PubMed]
4. Nishimura K, Nakagawa T, Ono K, Ogita H, Sakamoto T, Yamamoto N, Okita K, Yamanaka S, Ito J. Transplantation of mouse induced pluripotent stem cells into the cochlea. Neuroreport. 2009 Sep 23;20(14):1250-4. [CrossRef] [PubMed]
5. Rivolta MN. New strategies for the restoration of hearing loss: challenges and opportunities. Br Med Bull. 2013;105:69-84. [CrossRef] [PubMed]
6. Bozhkova,VP, Brezhestovsky LA, Buravlev VM. Rukovodstvo po kul’tivirovaniyu tkani. Metody. Tekhnika. Problemy. Moscow: Nauka; 1988. Russian.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Irina I. Sapizhak, Hryhoriy E. Timen, Vira M. Semenova, Lyudmyla P. Stayno
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ukrainian Neurosurgical Journal abides by the CREATIVE COMMONS copyright rights and permissions for open access journals.
Authors, who are published in this Journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the Journal under the terms of Creative Commons Attribution License, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this Journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form of which it has been published by the Journal (for example, to upload the work to the online storage of the Journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this Journal is included.
