Electroneuromyographic correlates of sciatic nerve function restoration after its resection and welded epineural coaptation in the experiment

Vitaliy I. Tsymbaliuk, Vitaliy Y. Molotkovets, Volodymyr V. Medvediev, Borys M. Luzan, Lesia S. Turuk, Мykhaylo М. Tatarchuk, Natalya G. Draguntsova

Abstract


Objective: To estimate the effectiveness of welding epineural coaptation of the residual sciatic nerve after resection based on electroneuromyographic (ENMG) parameters obtained in the gastrocnemius muscle.

Materials and methods. Experimental animals were albino outbreed male rats (350–450 g, 7 months old); trauma model was the resection of the left sciatic nerve in the middle third; the experimental groups were as following: 1 — neurotomy (n = 18), 2 — neurotomy + neurosuture (n = 13), 3 — neurotomy + welding coaptation (n = 15); the method of investigation was direct needle ENMG (sciatic nerve stimulation, responses were registered in gastrocnemius muscle) in 3 and 5 months after injury.

Results. The model of the nerve trauma with a temporary restriction of limb mobility is relevant for evaluating the effectiveness of restorative interventions in this type of pathology. In 5 months of observation there was found a significant prevalence of M-response amplitude in the injured limb compared to neurorrhaphy (17.3 ± 2.3 vs. 8.4 ± 0.9 mV, respectively; p = 0.005). M-response amplitude lateralization after the welded coaptation, in contrast to neurorrhaphy, is of temporary nature, indicating the improved regeneration process. Absence of ENMG-indices lateralization in 3 and 5 months after the neurotomy and high values of the M-response amplitude in 5 months indicated the possibility of gastrocnemius alternative re-innervations by terminals of intact nerve trunks.

Conclusion. High-frequency electric epineural welding provides a reliable coaptation of the residual nerve, and, taking into account some ENMG indicators, is more effective than neurorrhaphy.


Keywords


neurotomy; neurorrhaphy; welding coaptation of biological tissues; electroneuromyography; peripheral nerve regeneration

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DOI: https://doi.org/10.25305/unj.104503

Copyright (c) 2017 Vitaliy I. Tsymbaliuk, Vitaliy Y. Molotkovets, Volodymyr V. Medvediev, Borys M. Luzan, Lesia S. Turuk, Мykhaylo М. Tatarchuk, Natalya G. Draguntsova

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